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FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases.

ABSTRACT: A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.

SUBMITTER: Zhu J

PROVIDER: S-EPMC7007224 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases.

Zhu Jinbo J Haynes Cally J E CJE Kieffer Marion M Greenfield Jake L JL Greenhalgh Ryan D RD Nitschke Jonathan R JR Keyser Ulrich F UF

Journal of the American Chemical Society 20190711 29

A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we devel ...[more]

PMID: 31283214

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