Ontology highlight
ABSTRACT:
SUBMITTER: Galazzo L
PROVIDER: S-EPMC7007536 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Galazzo Laura L Meier Gianmarco G Timachi M Hadi MH Hutter Cedric A J CAJ Seeger Markus A MA Bordignon Enrica E
Proceedings of the National Academy of Sciences of the United States of America 20200121 5
Nanobodies are emerging tools in a variety of fields such as structural biology, cell imaging, and drug discovery. Here we pioneer the use of their spin-labeled variants as reporters of conformational dynamics of membrane proteins using DEER spectroscopy. At the example of the bacterial ABC transporter TM287/288, we show that two gadolinium-labeled nanobodies allow us to quantify, via analysis of the modulation depth of DEER traces, the fraction of transporters adopting the outward-facing state ...[more]