Dataset Information

Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.

ABSTRACT: CONTEXT:The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations. OBJECTIVE:To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3). DESIGN:LC-MS/MS method development and construction of estrogen reference ranges. SETTINGS:Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas. PARTICIPANTS:Healthy participants aged 3 months to 61 years (n = 1838). RESULTS:An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from

SUBMITTER: Frederiksen H

PROVIDER: S-EPMC7007877 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.

Frederiksen Hanne H Johannsen Trine Holm TH Andersen Stine Ehlern SE Albrethsen Jakob J Landersoe Selma Kløve SK Petersen Jørgen Holm JH Andersen Anders Nyboe AN Vestergaard Esben Thyssen ET Schorring Mia Elbek ME Linneberg Allan A Main Katharina M KM Andersson Anna-Maria AM Juul Anders A

The Journal of clinical endocrinology and metabolism 20200301 3

<h4>Context</h4>The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations.<h4>Objective</h4>To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3).<h4>Design</h4>LC-MS/MS method development and ...[more]

PMID: 31720688

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Dataset Information

Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.

Publications

Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.

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