Ontology highlight
ABSTRACT:
SUBMITTER: Des Soye BJ
PROVIDER: S-EPMC7008506 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Cell chemical biology 20191106 12
The site-specific incorporation of non-canonical amino acids (ncAAs) into proteins via amber suppression provides access to novel protein properties, structures, and functions. Historically, poor protein expression yields resulting from release factor 1 (RF1) competition has limited this technology. To address this limitation, we develop a high-yield, one-pot cell-free platform for synthesizing proteins bearing ncAAs based on genomically recoded Escherichia coli lacking RF1. A key feature of thi ...[more]