Dataset Information

Ammonium Transporter (BcAMT1.2) Mediates the Interaction of Ammonium and Nitrate in Brassica campestris.

ABSTRACT: The provision of ammonium (NH4 +) and nitrate (NO3 -) mixture increases the total nitrogen (N) than the supply of sole NH4 + or NO3 - with the same concentration of total N; thus, the mixture contributes to better growth in Brassica campestris. However, the underlying mechanisms remain unknown. In this study, we analyzed NH4 + and NO3 - fluxes using a scanning ion-selective electrode technique to detect under different N forms and levels in B. campestris roots. We observed that the total N influxes with NH4 + and NO3 - mixture were 1.25- and 3.53-fold higher than those with either sole NH4 + or NO3 -. Furthermore, NH4 + and NO3 - might interact with each other under coexistence. NO3 - had a positive effect on net NH4 + influx, whereas NH4 + had a negative influence on net NO3 - influx. The ammonium transporter (AMT) played a key role in NH4 + absorption and transport. Based on expression analysis, BcAMT1.2 differed from other BcAMT1s in being upregulated by NH4 + or NO3 -. According to sequence analysis and functional complementation in yeast mutant 31019b, AMT1.2 from B. campestris may be a functional AMT. According to the expression pattern of BcAMT1.2, ?-glucuronidase activity, and the cellular location of its promoter, BcAMT1.2 may be responsible for NH4 + transport. Following the overexpression of BcAMT1.2 in Arabidopsis, BcAMT1.2-overexpressing lines grew better than wildtype lines at low NH4 + concentration. In the mixture of NH4 + and NO3 -, NH4 + influxes and NO3 - effluxes were induced in BcAMT1.2-overexpressing lines. Furthermore, transcripts of N assimilation genes (AtGLN1.2, AtGLN2, and AtGLT1) were significantly upregulated, in particular, AtGLN1.2 and AtGLT1 were increased by 2.85-8.88 times in roots, and AtGLN1.2 and AtGLN2 were increased by 2.67-4.61 times in leaves. Collectively, these results indicated that BcAMT1.2 may mediate in NH4 + fluxes under the coexistence of NH4 + and NO3 - in B. campestris.

SUBMITTER: Zhu Y

PROVIDER: S-EPMC7011105 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Ammonium Transporter (BcAMT1.2) Mediates the Interaction of Ammonium and Nitrate in Brassica campestris.

Zhu Yunna Y Huang Xinmin X Hao Yanwei Y Su Wei W Liu Houcheng H Sun Guangwen G Chen Riyuan R Song Shiwei S

Frontiers in plant science 20200204

The provision of ammonium (NH4 +) and nitrate (NO3 -) mixture increases the total nitrogen (N) than the supply of sole NH4 + or NO3 - with the same concentration of total N; thus, the mixture contributes to better growth in Brassica campestris. However, the underlying mechanisms remain unknown. In this study, we analyzed NH4 + and NO3 - fluxes using a scannin ...[more]

PMID: 32117342

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Project description:BackgroundPlantlets grown in vitro with a mixed nitrogen source utilize sucrose and CO2 as carbon sources for growth. However, it is very difficult to obtain the correct utilization proportions of nitrate, ammonium, sucrose and CO2 for plantlets. Consequently, the biological effect of ammonium/nitrate utilization, the biological effect of sucrose/CO2 utilization, and the ammonium/nitrate use efficiency for new C input derived from CO2 assimilation/sucrose utilization are still unclear for plantlets.ResultsThe bidirectional stable nitrogen isotope tracer technique quantified the proportions of assimilated nitrate and ammonium in Brassica napus plantlets grown at different ammonium/nitrate ratios. The utilization proportions of sucrose and CO2 could be quantified by a two end-member isotope mixing model for Bn plantlets grown at different ammonium/nitrate ratios. Under the condition that each treatment contained 20 mM ammonium, the proportion of assimilated nitrate did not show a linear increase with increasing nitrate concentration for Bn plantlets. Moreover, the proportion of assimilated CO2 did not show a linear relationship with the nitrate concentration for Bn plantlets. Increasing the nitrate concentration contributed to promoting the assimilation of ammonium and markedly enhanced the ammonium utilization coefficient for Bn plantlets. With increasing nitrate concentration, the amount of nitrogen in leaves derived from nitrate assimilation increased gradually, while the nitrate utilization coefficient underwent no distinct change for Bn plantlets.ConclusionsQuantifying the utilization proportions of nitrate and ammonium can reveal the energy efficiency for N assimilation in plantlets grown in mixed N sources. Quantifying the utilization proportion of CO2 contributes to evaluating the photosynthetic capacity of plantlets grown with variable ammonium/nitrate ratios. Quantifying the utilization proportions of nitrate, ammonium, sucrose and CO2 can reveal the difference in the ammonium/nitrate use efficiency for new C input derived from CO2 assimilation/sucrose utilization for plantlets grown at variable ammonium/nitrate ratios.

Dataset Information

Ammonium Transporter (BcAMT1.2) Mediates the Interaction of Ammonium and Nitrate in Brassica campestris.

Publications

Ammonium Transporter (<i>BcAMT1.2</i>) Mediates the Interaction of Ammonium and Nitrate in <i>Brassica campestris</i>.

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