Dataset Information

An in situ-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce.

ABSTRACT: Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people's life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific sequences of Salmonella Typhimurium, Vibrio parahemolyticus, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7 from the National Center for Biotechnology Information database. Tiling array probes were designed to target selected genes in an optimized hybridization system. A total of 141 specific probes were selected from 3,227 hybridization probes, comprising 26 L. monocytogenes, 24 S. aureus, 25 E. coli O157:H7, 20 Salmonella Typhimurium, and 46 V. parahemolyticus probes that are unique to this study. The optimized assay had strong amplification signals and high accuracy. The detection limit for the five target pathogens on fresh-cut cantaloupe and lettuce was approximately 3 log cfu/g without culturing and with a detection time of 24 h. The detection technology established in this study can rapidly detect and monitor the foodborne pathogens on fresh-cut fruits and vegetables throughout the logistical distribution chain, i.e., processing, cleaning, fresh-cutting, packaging, storage, transport, and sale, and represents a valuable technology that support the safety of fresh agricultural products.

SUBMITTER: Sarengaowa

PROVIDER: S-EPMC7012807 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

An in situ-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce.

Sarengaowa Hu Wenzhong W Feng Ke K Jiang Aili A Xiu Zhilong Z Lao Ying Y Li Yuanzheng Y Long Ya Y

Frontiers in microbiology 20200205

Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people's life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific sequences of Salmonella Typhimurium, Vibrio parahemolyticus, Staphylococcus a ...[more]

PMID: 32117079

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Project description:Visual assessments are used for evaluating the quality of food products, such as fresh-cut lettuce packaged in bags with modified atmosphere. We have compared the accuracy and the reliability of visual evaluations of decay on fresh-cut lettuce performed with experienced and inexperienced raters. In addition, we have analyzed decay data from over 4.5 thousand bags to determine the optimum timing for evaluations to detect differences among accessions. Lin's concordance coefficient (?c) that takes into consideration both the closeness of the data and the conformance to the identity line showed high repeatability (intra-rater reliability, ?c = 0.97), reproducibility (inter-rater reliability, ?c = 0.92), and accuracy (?c = 0.96) for experienced raters. Inexperienced raters did not perform as well and their ratings showed decreased repeatability (?c = 0.93), but even larger reduction in reproducibility (?c = 0.80) and accuracy (?c = 0.90). We have detected that 5.3% of ratings were outside of the 95% limits of agreement. These under- or overestimates were predominantly found for bags with intermediate levels of decay, which corresponds to the middle of the rating scale. This occurs because intermediate amounts of decay are more difficult to discriminate than extremes. The frequencies of aberrant ratings for experienced raters ranged from 0.6% to 4.4% (mean = 2.1%), for inexperienced raters the frequencies were substantially higher, ranging from 6.1% to 15.6% (mean = 9.4%). Therefore, we recommend that new raters receive training that includes practical examples in this range of decay, use of standard area diagrams, and continuing interaction with experienced raters (consultation during actual rating). Very high agreement among experienced raters indicate that visual ratings can be successfully used for evaluations of decay, until a more objective, rapid, and affordable method is developed. We recommend evaluating samples at multiple time points until 42 days after processing (about 80% decay on average) and then combining these individual ratings into the area under the decay progress stairs (AUDePS) score. Applying this approach, experienced evaluators can accurately detect difference among lettuce accessions and identify lettuce cultivars with reduced decay.

Dataset Information

An in situ-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce.

Publications

An <i>in situ</i>-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce.

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