Dataset Information

Fifteen-Year Application of Manure and Chemical Fertilizers Differently Impacts Soil ARGs and Microbial Community Structure.

ABSTRACT: Manure, which contains large amounts of antibiotics and antibiotic resistance genes (ARGs), is widely used in agricultural soils and may lead to the evolution and dispersal of ARGs in the soil environment. In the present study, soils that received manure or chemical fertilizers for 15 years were sampled on the North China Plain (NCP), which is one of the primary areas of intensive agriculture in China. High-throughput quantitative PCR and sequencing technologies were employed to assess the effects of long-term manure or chemical fertilizer application on the distribution of ARGs and microbial communities. A total of 114 unique ARGs were successfully amplified from all soil samples. Manure application markedly increased the relative abundance and detectable numbers of ARGs, with up to 0.23 copies/16S rRNA gene and 81 unique ARGs. The increased abundance of ARGs in manure-fertilized soil was mainly due to the manure increasing the abundance of indigenous soil ARGs. In contrast, chemical fertilizers only moderately affected the diversity of ARGs and had no significant effect on the relative abundance of the total ARGs. In addition, manure application increased the abundance of mobile genetic elements (MGEs), which were significantly and positively correlated with most types of ARGs, indicating that horizontal gene transfer via MGEs may play an important role in the spread of ARGs. Furthermore, the application of manure and chemical fertilizers significantly affected microbial community structure, and variation partitioning analysis showed that microbial community shifts represented the major driver shaping the antibiotic resistome. Taken together, our results provide insight into the long-term effects of manure and chemical fertilization on the dissemination of ARGs in intensive agricultural ecosystems.

SUBMITTER: Wang F

PROVIDER: S-EPMC7015874 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Fifteen-Year Application of Manure and Chemical Fertilizers Differently Impacts Soil ARGs and Microbial Community Structure.

Wang Fenghua F Han Wanxue W Chen Shuaimin S Dong Wenxu W Qiao Min M Hu Chunsheng C Liu Binbin B

Frontiers in microbiology 20200206

Manure, which contains large amounts of antibiotics and antibiotic resistance genes (ARGs), is widely used in agricultural soils and may lead to the evolution and dispersal of ARGs in the soil environment. In the present study, soils that received manure or chemical fertilizers for 15 years were sampled on the North China Plain (NCP), which is one of the primary areas of intensive agriculture in China. High-throughput quantitative PCR and sequencing technologies were employed to assess the effec ...[more]

PMID: 32117108

Similar Datasets

Project description:Soil microorganisms play vital roles in energy flow and soil nutrient cycling and, thus, are important for crop production. A detailed understanding of the complex responses of microbial communities to diverse organic manure and chemical fertilizers (CFs) is crucial for agroecosystem sustainability. However, little is known about the response of soil fungal communities and soil nutrients to manure and CFs, especially under double-rice cropping systems. In this study, we investigated the effects of the application of combined manure and CFs to various fertilization strategies, such as no N fertilizer (Neg-CF); 100% chemical fertilizer (Pos-CF); 60% cattle manure (CM) + 40% CF (high-CM); 30% CM + 70% CF (low-CM); 60% poultry manure (PM) + 40% CF (high-PM), and 30% PM + 70% CF (low-PM) on soil fungal communities' structure and diversity, soil environmental variables, and rice yield. Results showed that synthetic fertilizer plus manure addition significantly increased the soil fertility and rice grain yield compared to sole CFs' application. Moreover, the addition of manure significantly changed the soil fungal community structure and increased the relative abundance of fungi such as phyla Ascomycota, Basidiomycota, Mortierellomycota, and Rozellomycota. The relative abundances dramatically differed at each taxonomic level, especially between manured and non-manured regimes. Principal coordinates analysis (PCoA) exhibited greater impacts of the addition of manure amendments than CFs on fungal community distributions. Redundancy analysis showed that the dominant fungal phyla were positively correlated with soil pH, soil organic C (SOC), total N, and microbial biomass C, and the fungal community structure was strongly affected by SOC. Network analysis explored positive relationships between microorganisms and could increase their adaptability in relevant environments. In addition, the structural equation model (SEM) shows the relationship between microbial biomass, soil nutrients, and rice grain yield. The SEM showed that soil nutrient contents and their availability directly affect rice grain yield, while soil fungi indirectly affect grain yield through microbial biomass production and nutrient levels. Our results suggest that manure application combined with CFs altered soil biochemical traits and soil fungal community structure and counteracted some of the adverse effects of the synthetic fertilizer. Overall, the findings of this research suggest that the integrated application of CF and manure is a better approach for improving soil health and rice yield.

Project description:Biostimulants are gaining momentum as potential soil amendments to increase plant health and productivity. Plant growth responses to some biostimulants and poorly soluble fertilizers could increase soil microbial diversity and provide greater plant access to less soluble nutrients. We assessed an agricultural soil amended with a multispecies microbial biostimulant in comparison with two fertilizers that differed in elemental solubilities to identify effects on soil bacterial communities associated with two annual pasture species (subterranean clover and Wimmera ryegrass). The treatments applied were: a multispecies microbial biostimulant, a poorly soluble rock mineral fertilizer at a rate of 5.6 kg P ha-1, a chemical fertilizer at a rate of 5.6 kg P ha-1, and a negative control with no fertilizer or microbial biostimulant. The two annual pasture species were grown separately for 10 weeks in a glasshouse with soil maintained at 70% of field capacity. Soil bacteria were studied using 16S rRNA with 27F and 519R bacterial primers on the Mi-seq platform. The microbial biostimulant had no effect on growth of either of the pasture species. However, it did influence soil biodiversity in a way that was dependent on the plant species. While application of the fertilizers increased plant growth, they were both associated with the lowest diversity of the soil bacterial community based on Fisher and Inverse Simpson indices. Additionally, these responses were plant-dependent; soil bacterial richness was highly correlated with soil pH for subterranean clover but not for Wimmera ryegrass. Soil bacterial richness was lowest following application of each fertilizer when subterranean clover was grown. In contrast, for Wimmera ryegrass, soil bacterial richness was lowest for the control and rock mineral fertilizer. Beta diversity at the bacterial OTU level of resolution by permanova demonstrated a significant impact of soil amendments, plant species and an interaction between plant type and soil amendments. This experiment highlights the complexity of how soil amendments, including microbial biostimulants, may influence soil bacterial communities associated with different plant species, and shows that caution is required when linking soil biodiversity to plant growth. In this case, the microbial biostimulant influenced soil biodiversity without influencing plant growth.

Dataset Information

Fifteen-Year Application of Manure and Chemical Fertilizers Differently Impacts Soil ARGs and Microbial Community Structure.

Publications

Fifteen-Year Application of Manure and Chemical Fertilizers Differently Impacts Soil ARGs and Microbial Community Structure.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets