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Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis.

ABSTRACT: Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. However, isolating every single compound from a natural extract is not always possible due to the complex chemistry and presence of most secondary metabolites at very low levels. Metabolomics has emerged in recent years as an indispensable tool for the analysis of thousands of metabolites from crude natural extracts, leading to a paradigm shift in natural products drug research. Analytical methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) are used to comprehensively annotate the constituents of plant natural products for screening, drug discovery as well as for quality control purposes such as those required for phytomedicine. In this review, the current advancements in plant sample preparation, sample measurements, and data analysis are presented alongside a few case studies of the successful applications of these processes in plant natural product drug discovery.

SUBMITTER: Salem MA

PROVIDER: S-EPMC7023240 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis.

Salem Mohamed A MA Salem Mohamed A MA Perez de Souza Leonardo L Serag Ahmed A Fernie Alisdair R AR Farag Mohamed A MA Ezzat Shahira M SM Alseekh Saleh S

Metabolites 20200115 1

Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. However, isolating every single compound from a natural extract is not always possible due to the complex chemistry and presence of most secondary metabolites at very low levels. Metabolomics has emerg ...[more]

PMID: 31952212

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Dataset Information

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis.

Publications

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis.

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