Ontology highlight
ABSTRACT:
SUBMITTER: Bucholc K
PROVIDER: S-EPMC7026596 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Bucholc Katarzyna K Aik Wei Shen WS Yang Xiao-Cui XC Wang Kaituo K Zhou Z Hong ZH Dadlez Michał M Marzluff William F WF Tong Liang L Dominski Zbigniew Z
Nucleic acids research 20200201 3
In animal cells, replication-dependent histone pre-mRNAs are cleaved at the 3' end by U7 snRNP consisting of two core components: a ∼60-nucleotide U7 snRNA and a ring of seven proteins, with Lsm10 and Lsm11 replacing the spliceosomal SmD1 and SmD2. Lsm11 interacts with FLASH and together they recruit the endonuclease CPSF73 and other polyadenylation factors, forming catalytically active holo U7 snRNP. Here, we assembled core U7 snRNP bound to FLASH from recombinant components and analyzed its ap ...[more]