Ontology highlight
ABSTRACT:
SUBMITTER: Wang T
PROVIDER: S-EPMC7028383 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Wang Tianyu T Wu Chunyan C Ouzounov Dimitre G DG Gu Wenchao W Xia Fei F Kim Minsu M Yang Xusan X Warden Melissa R MR Xu Chris C
eLife 20200130
1300 nm three-photon calcium imaging has emerged as a useful technique to allow calcium imaging in deep brain regions. Application to large-scale neural activity imaging entails a careful balance between recording fidelity and perturbation to the sample. We calculated and experimentally verified the excitation pulse energy to achieve <i>the minimum photon count</i> required for the detection of calcium transients in GCaMP6s-expressing neurons for 920 nm two-photon and 1320 nm three-photon excita ...[more]