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Polyurethane/polycaprolactone membrane grafted with conjugated linoleic acid for artificial vascular graft application.

ABSTRACT: Constructing satisfied small-diameter vascular graft (diameter less than 6 mm) remains an unsolvable challenge in vascular tissue engineering. This study described the fabrication of electrospun polyurethane/polycaprolactone (PU/PCL) membranes chemically grafted with various densities of conjugated linoleic acid (CLA) - an antithrombotic fatty acid - for making small-diameter blood vessel. Differences in mechanical, antithrombotic properties and biocompatibility of the membranes resulting from the CLA-grafting procedure were the focus of the study. Investigation of mechanical properties relevant to vascular graft application revealed that these properties of the membranes remained unaffected and satisfied clinical criteria following the CLA graft. Blood-membrane interaction assays showed that the CLA-grafted membranes mitigated the adhesion of blood cells, as well as preventing blood coagulation. These effects were also commensurate with increasing density of CLA, suggesting an effective approach to improve antithromboticity. Cellular tests suggested that CLA has an optimal density at which it promoted cell proliferation on the surface of the membranes; however, excessive presence of CLA might cause undesirable inhibition on cells. In conclusion, PU/PCL membrane grafted with CLA could be a prospective material for vascular tissue engineering with further development and investigation.

SUBMITTER: Tran N

PROVIDER: S-EPMC7033711 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Wavy small-diameter vascular graft made of eggshell membrane and thermoplastic polyurethane.

Project description:In this study, a small-diameter, double-layered eggshell membrane/thermoplastic polyurethane (ESM/TPU) vascular graft with a wavy structure was developed. The avian eggshell membrane, a fibrous structure similar to the extracellular matrix (ECM), has the potential to yield rapid endothelialization in vitro. The dopamine and heparin modification of the ESM surface not only promoted human umbilical vein endothelial cell (HUVEC) proliferation via cytocompatibility assessment, but also improved its anticoagulation properties as verified in platelet adhesion tests. The biomimetic mechanical properties of the vascular graft were provided by the elastic TPU fibers via electrospinning using a wavy cross-section rotating collector. The advantage of combining these two materials is to make use of the bioactivity of ESM as the internal membrane and the tunable mechanical properties of TPU as the external layer. The circumferentially wavy structure of the vascular graft produced a toe region in the non-linear section of the stress-strain curve similar to that of natural blood vessels. The ESM/TPU graft's circumferential ultimate strength was 2.57?MPa, its strain was 339% mm/mm, and its toe region was found to be around 20% mm/mm. Cyclical tension tests showed that the vascular graft could maintain good mechanical properties and showed no structural damage under repeated extension tests.

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Project description:Biodegradable synthetic polymers have been widely used as tissue engineering scaffold materials. Even though they have shown excellent biocompatibility, they have failed to resemble the low stiffness and high elasticity of soft tissues because of the presence of massive rigid ester bonds. Herein, we synthesized a new thermoplastic polyurethane elastomer (CTC-PU(BET)) using poly ester ether triblock copolymer (polycaprolactone-block-polytetrahydrofuran-block-polycaprolactone triblock copolymer, PCTC) as the soft segment, aliphatic diisocyanate (hexamethylene diisocyanate, HDI) as the hard segment, and degradable diol (bis(2-hydroxyethyl) terephthalate, BET) as the chain extender. PCTC inhibited crystallization and reduced the melting temperature of CTC-PU(BET), and BET dramatically enhanced the thermal decomposition and hydrolytic degradation rate when compared with conventional polyester-based biodegradable TPUs. The CTC-PU(BET) synthesized in this study possessed a low tensile modulus and tensile strength of 2.2 MPa and 1.3 MPa, respectively, and an elongation-at-break over 700%. Meanwhile, it maintained a 95.3% recovery rate and 90% resilience over ten cycles of loading and unloading. In addition, the TPU could be electrospun into both random and aligned fibrous scaffolds consisting of major microfibers and nanobranches. 3T3 fibroblast cell culture confirmed that these scaffolds outperformed the conventional biodegradable TPU scaffolds in terms of substrate-cellular interactions and cell proliferation. Considering the advantages of this TPU, such as ease of synthesis, low cost, low stiffness, high elasticity, controllable degradation rate, ease of processability, and excellent biocompatibility, it has great prospects to be used as a tissue engineering scaffold material for soft tissue regeneration.

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Conjugated linoleic acid (CLA) and Caco-2 cells

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2007-12-13 | GSE6518 | GEO

Polyvinyl alcohol coating prevents platelet adsorption and improves mechanical property of polycaprolactone-based small-caliber vascular graft.

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