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Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.


ABSTRACT: Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent "helper" adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (?G) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.

SUBMITTER: Lavin TK 

PROVIDER: S-EPMC7033752 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.

Lavin Thomas K TK   Jin Lei L   Lea Nicholas E NE   Wickersham Ian R IR  

Frontiers in synaptic neuroscience 20200214


Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent "helper" adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generat  ...[more]

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