Model-Driven Engineering of N-Linked Glycosylation in Chinese Hamster Ovary Cells.
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ABSTRACT:
SUBMITTER: Stach CS
PROVIDER: S-EPMC7034315 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Model-Driven Engineering of N-Linked Glycosylation in Chinese Hamster Ovary Cells.
Stach Christopher S CS McCann Meghan G MG O'Brien Conor M CM Le Tung S TS Somia Nikunj N Chen Xinning X Lee Kyoungho K Fu Hsu-Yuan HY Daoutidis Prodromos P Zhao Liang L Hu Wei-Shou WS Smanski Michael M
ACS synthetic biology 20191018 11
Chinese hamster ovary (CHO) cells are used for industrial production of protein-based therapeutics (i.e., "biologics"). Here we describe a method for combining systems-level kinetic models with a synthetic biology platform for multigene overexpression to rationally perturb N-linked glycosylation. Specifically, we sought to increase galactose incorporation on a secreted Immunoglobulin G (IgG) protein. We rationally design, build, and test a total of 23 transgenic cell pools that express single or ...[more]
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