Dataset Information

Genome-wide identification, expression, and association analysis of the monosaccharide transporter (MST) gene family in peanut (Arachis hypogaea L.).

ABSTRACT: In this study, we reported the genome-wide analysis of the whole sugar transporter gene family of a legume species, peanut (Arachis hypogaea L.), including the chromosome locations, gene structures, phylogeny, expression patterns, as well as comparative genomic analysis with Arabidopsis, rice, grape, and soybean. A total of 76 AhMST genes (AhMST1-76) were identified from the peanut genome and located unevenly in 20 chromosomes. Phylogeny analysis indicated that the AhMSTs can be divided into eight groups including two undefined peanut-specific groups. Transcriptional profiles revealed that many AhMST genes showed tissue-specific expression, the majority of the AhMST genes mainly expressed in sink organs and floral organ of peanut. Chromosome distribution pattern and synteny analysis strongly indicated that genome-wide segmental and tandem duplication contributed to the expansion of peanut MST genes. Four common orthologs (AhMST9, AhMST13, AhMST40, and AhMST43) between peanut and the other four species were identified by comparative genomic analysis, which might play important roles in maintaining the growth and development of plant. Furthermore, four polymorphic sites in AhMST11, AhMST13, and AhMST60 were significantly correlated with hundred pod weight (HPW) and hundred seed weight (HSW) by association analysis. In a word, these results will provide new insights for understanding the functions of AhMST family members to sugar transporting and the potential for yield improvement in peanut.

SUBMITTER: Wan L

PROVIDER: S-EPMC7035416 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Genome-wide identification, expression, and association analysis of the monosaccharide transporter (MST) gene family in peanut (Arachis hypogaea L.).

Wan Liyun L Ren Weifang W Miao Haocui H Zhang Juncheng J Fang Jiahai J

3 Biotech 20200221 3

In this study, we reported the genome-wide analysis of the whole sugar transporter gene family of a legume species, peanut (Arachis hypogaea L.), including the chromosome locations, gene structures, phylogeny, expression patterns, as well as comparative genomic analysis with Arabidopsis, rice, grape, and soybean. A total of 76 AhMST genes (AhMST1-76) were identified from the peanut genome and located unevenly in 20 chromosomes. Phylogeny analysis indicated that the AhMSTs can be di ...[more]

PMID: 32154043

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Project description:BACKGROUND:Microsatellites, or simple sequence repeats (SSRs), represent important DNA variations that are widely distributed across the entire plant genome and can be used to develop SSR markers, which can then be used to conduct genetic analyses and molecular breeding. Cultivated peanut (A. hypogaea L.), an important oil crop worldwide, is an allotetraploid (AABB, 2n = 4× = 40) plant species. Because of its complex genome, genomic marker development has been very challenging. However, sequencing of cultivated peanut genome allowed us to develop genomic markers and construct a high-density physical map. RESULTS:A total of 8,329,496 SSRs were identified, including 3,772,653, 4,414,961, and 141,882 SSRs that were distributed in subgenome A, B, and nine scaffolds, respectively. Based on the flanking sequences of the identified SSRs, a total of 973,984 newly developed SSR markers were developed in subgenome A (462,267), B (489,394), and nine scaffolds (22,323), with an average density of 392.45 markers per Mb. In silico PCR evaluation showed that an average of 88.32% of the SSR markers generated only one in silico-specific product in two tetraploid A. hypogaea varieties, Tifrunner and Shitouqi. A total of 39,599 common SSR markers were identified among the two A. hypogaea varieties and two progenitors, A. duranensis and A. ipaensis. Additionally, an amplification effectiveness of 44.15% was observed by real PCR validation. Moreover, a total of 1276 public SSR loci were integrated with the newly developed SSR markers. Finally, a previously known leaf spot quantitative trait locus (QTL), qLLS_T13_A05_7, was determined to be in a 1.448-Mb region on chromosome A05. In this region, a total of 819 newly developed SSR markers were located and 108 candidate genes were detected. CONCLUSIONS:The availability of these newly developed and public SSR markers both provide a large number of molecular markers that could potentially be used to enhance the process of trait genetic analyses and improve molecular breeding strategies for cultivated peanut.

Dataset Information

Genome-wide identification, expression, and association analysis of the monosaccharide transporter (MST) gene family in peanut (Arachis hypogaea L.).

Publications

Genome-wide identification, expression, and association analysis of the monosaccharide transporter (MST) gene family in peanut (<i>Arachis hypogaea</i> L.).

Similar Datasets

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