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Virus Isolation from the First Patient with SARS-CoV-2 in Korea.

ABSTRACT: Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

SUBMITTER: Park WB

PROVIDER: S-EPMC7036342 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Virus Isolation from the First Patient with SARS-CoV-2 in Korea.

Park Wan Beom WB Kwon Nak Jung NJ Choi Su Jin SJ Kang Chang Kyung CK Choe Pyoeng Gyun PG Kim Jin Yong JY Yun Jiyoung J Lee Gir Won GW Seong Moon Woo MW Kim Nam Joong NJ Seo Jeong Sun JS Oh Myoung Don MD

Journal of Korean medical science 20200224 7

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coro ...[more]

PMID: 32080990

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