Dataset Information

Virulence Constitution of Multi-Drug-Resistant Pseudomonas aeruginosa in Upper Egypt.

ABSTRACT: Purpose:Ventilator-associated pneumonia caused by Pseudomonas aeruginosa (P. aeruginosa) is a major health-care problem. In this study, we explored the epidemiology of virulence determinants among multi-drug-resistant (MDR) clinical P. aeruginosa isolates from hospitalized patients with ventilator-associated pneumonia in intensive care units in Upper Egypt. Patients and Methods:MDR P. aeruginosa isolates were screened for the presence of eight virulence factors and typed by ERIC-PCR. Results:A total of 39 clinical MDR isolates were selected out of 173 isolated P. aeruginosa showing a combination of adhesion and cytotoxicity virulence patterns, with the detection of aprA, exoU, exoS, lasB, algD, toxA in 74.3%, 58.9%, 46.1%, 41.2%, 30.7%, 20.5% of the isolates, respectively. The MDR isolates were grouped into 13 different virulence profiles according to the pattern of virulence gene distribution. exoU genotype was more predominant among the P. aeruginosa isolates with more than 48% of the isolates harboring this gene alone, 7% harboring both exoU and exoS and 43.5% harboring exoS gene. An intermediate degree of diversity was detected by ERIC-PCR typing where the isolates were clustered in 7 major groups, indicating possible cross-infection within the hospital. Conclusion:Our results highlight the increased frequency of virulent P. aeruginosa isolates with a shift to the more virulent cytotoxic exoU genotype. Further hospital infection-control measures are mandatory to control the hospital cross-transmission of these highly virulent isolates. This study could vastly be a help to develop efficient treatment policies against P. aeruginosa induced ventilator-associated pneumonia.

SUBMITTER: Hassuna NA

PROVIDER: S-EPMC7036984 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Virulence Constitution of Multi-Drug-Resistant <i>Pseudomonas aeruginosa</i> in Upper Egypt.

Hassuna Noha A NA Mandour Sahar A SA Mohamed Ebtisam Samir ES

Infection and drug resistance 20200219

<h4>Purpose</h4>Ventilator-associated pneumonia caused by <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is a major health-care problem. In this study, we explored the epidemiology of virulence determinants among multi-drug-resistant (MDR) clinical <i>P. aeruginosa</i> isolates from hospitalized patients with ventilator-associated pneumonia in intensive care units in Upper Egypt.<h4>Patients and methods</h4>MDR <i>P. aeruginosa</i> isolates were screened for the presence of eight virulence ...[more]

PMID: 32110069

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Project description:The increasing prevalence of multi-drug-resistant (MDR) strains of Pseudomonas aeruginosa among critically ill humans is of significant concern. In the current study, we show that MDR clinical isolates of P. aeruginosa representing three distinct genotypes that display high virulence against intestinal epithelial cells, form novel appendage-like structures on their cell surfaces. These appendages contain PstS, an extracellular phosphate binding protein. Using anti-PstS antibodies, we determined that the PstS-rich appendages in MDR strains are involved in adherence to and disruption of the integrity of cultured intestinal epithelial cell monolayers. The outer surface-expressed PstS protein was also identified to be present in P. aeruginosa MPAO1, although to a lesser degree, and its role in conferring an adhesive and barrier disruptive phenotype against intestinal epithelial cells was confirmed using an isogenic DeltaPstS mutant. Formation of the PstS rich appendages was induced during phosphate limitation and completely suppressed in phosphate-rich media. Injection of MDR strains directly into the intestinal tract of surgically injured mice, a known model of phosphate limitation, caused high mortality rates (60%-100%). Repletion of intestinal phosphate in this model completely prevented mortality. Finally, significantly less outer surface PstS was observed in the MPAO1 mutant DeltaHxcR thus establishing a role for the alternative type II secretion system Hxc in outer surface PstS expression. Gene expression analysis performed by RT-PCR confirmed this finding and further demonstrated abundant expression of pstS analogous to pa5369, pstS analogous to pa0688/pa14-55410, and hxcX in MDR strains. Taken together, these studies provide evidence that outer surface PstS expression confers a highly virulent phenotype of MDR isolates against the intestinal epithelium that alters their adhesive and barrier disrupting properties against the intestinal epithelium.

Dataset Information

Virulence Constitution of Multi-Drug-Resistant Pseudomonas aeruginosa in Upper Egypt.

Publications

Virulence Constitution of Multi-Drug-Resistant <i>Pseudomonas aeruginosa</i> in Upper Egypt.

Similar Datasets

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