Dataset Information

Bioassay-Guided Isolation and HPLC Quantification of Antiproliferative Metabolites from Stahlianthus Thorelii.

ABSTRACT: In folk medicine, Stahlianthus thorelii Gagnep. has been used to treat diseases related to inflammation, ulcers, and cancer. There are no reports concerning the chemical components and bioactivities of S. thorelii; thus, this study aims to explore the phytochemicals, quantify the main compounds, and test the anticancer activity of isolates from S. thorelii. Dried rhizomes were extracted with 95% ethanol and, then, partitioned, fractionated, and isolated. On the basis of the result of the antiproliferative activity of the fractions, seven isolates were yielded and were identified by spectroscopic analyses. The inhibition of cancer proliferation was determined by an MTT assay and the deployed IC50 to value their efficacy. Seven compounds containing one new C-benzylated dihydrochalcone derivative, thorechalcone A (1) and 2-7 were isolated from S. thorelii. In terms of the bioactivity, compounds 1 and 3 displayed promising antiproliferative activity (WiDr, A549, and HepG2), with IC50 values <40 µM. The HPLC-UV method of quantification of two major compounds (3 and 4) was also validated. This study presented the isolations of antiproliferative potentials of new chalcone and known flavonoid derivatives from S. thorelii. The validated simple, accurate, and rapid HPLC method could be deployed for the quality control of herbal drugs.

SUBMITTER: Nguyen NL

PROVIDER: S-EPMC7038115 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Bioassay-Guided Isolation and HPLC Quantification of Antiproliferative Metabolites from Stahlianthus Thorelii.

Nguyen Nham-Linh NL Vo Thanh-Hoa TH Lin Yu-Chi YC Liaw Chia-Ching CC Lin Zhi-Hu ZH Chen Mei-Chuan MC Kuo Yao-Haur YH

Molecules (Basel, Switzerland) 20200128 3

In folk medicine, Stahlianthus thorelii Gagnep. has been used to treat diseases related to inflammation, ulcers, and cancer. There are no reports concerning the chemical components and bioactivities of S. thorelii; thus, this study aims to explore the phytochemicals, quantify the main compounds, and test the anticancer activity of isolates from S. thorelii. Dried rhizomes were extracted with 95% ethanol and, then, partitioned, fractionated, and isolated. On the basis of the ...[more]

PMID: 32012805

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Dataset Information

Bioassay-Guided Isolation and HPLC Quantification of Antiproliferative Metabolites from Stahlianthus Thorelii.

Publications

Bioassay-Guided Isolation and HPLC Quantification of Antiproliferative Metabolites from <i>Stahlianthus Thorelii</i>.

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