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Genetic Factors Associated with Enhanced bla KPC Expression in Tn3/Tn4401 Chimeras.

ABSTRACT: The expression of the bla KPC gene plays a key role in carbapenem resistance in Enterobacteriaceae However, the genetic regulators of the bla KPC gene have not been completely elucidated, especially the genes in Tn3-Tn4401 chimeras. Two novel Tn3-Tn4401 chimera isoforms were characterized in our hospital, isoform A (CTA), which harbors a 121-bp deletion containing the PX promoter and was present in 22.6% (54/239) of isolates, and isoform C (CTC), which harbors a 624-bp insertion and a P1 promoter deletion and was present in only 1 isolate. The carbapenem MICs of both isoforms were 2-fold or more higher than those of the wild type (Tn3-Tn4401 chimera, CTB), and bla KPC was most highly expressed in CTA. Bioinformatics and 5' rapid amplification of cDNA ends (5' RACE) experiments indicated a novel strong putative promoter, PY, at the 3' end of the ISKpn8 gene. PY mutation nearly abrogated bla KPC expression (P?

SUBMITTER: Huang J 

PROVIDER: S-EPMC7038255 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Genetic Factors Associated with Enhanced <i>bla</i><sub>KPC</sub> Expression in Tn<i>3</i>/Tn<i>4401</i> Chimeras.

Huang Jiansheng J   Hu Xiaolei X   Zhao Yunan Y   Shi Yang Y   Ding Hui H   Xv Jianfen J   Ren Jianmin J   Wu Rongzhen R   Zhao Zhigang Z  

Antimicrobial agents and chemotherapy 20200221 3

The expression of the <i>bla</i><sub>KPC</sub> gene plays a key role in carbapenem resistance in <i>Enterobacteriaceae</i> However, the genetic regulators of the <i>bla</i><sub>KPC</sub> gene have not been completely elucidated, especially the genes in Tn<i>3</i>-Tn<i>4401</i> chimeras. Two novel Tn<i>3</i>-Tn<i>4401</i> chimera isoforms were characterized in our hospital, isoform A (CTA), which harbors a 121-bp deletion containing the PX promoter and was present in 22.6% (54/239) of isolates, a  ...[more]

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