Ontology highlight
ABSTRACT:
SUBMITTER: Meuris L
PROVIDER: S-EPMC7039703 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Meuris Leander L Santens Francis F Elson Greg G Festjens Nele N Boone Morgane M Dos Santos Anaëlle A Devos Simon S Rousseau François F Plets Evelyn E Houthuys Erica E Malinge Pauline P Magistrelli Giovanni G Cons Laura L Chatel Laurence L Devreese Bart B Callewaert Nico N
Nature biotechnology 20140420 5
Heterogeneity in the N-glycans on therapeutic proteins causes difficulties for protein purification and process reproducibility and can lead to variable therapeutic efficacy. This heterogeneity arises from the multistep process of mammalian complex-type N-glycan synthesis. Here we report a glycoengineering strategy--which we call GlycoDelete--that shortens the Golgi N-glycosylation pathway in mammalian cells. This shortening results in the expression of proteins with small, sialylated trisacchar ...[more]