Comparison of osteogenic and dentinogenic potentials of mice incisor and molar pulps in vitro.

ABSTRACT: OBJECTIVE:In the present study, we compared the in vitro osteogenic and dentinogenic potential of pulp cells from incisors and molars. DESIGN:Primary pulp cultures were established from DSPP-Cerulean/DMP1-Cherry and BSP-GFPtpz reporter mouse lines and processed for various assays. RESULTS:Our results showed marked differences in dentinogenic and osteogenic potentials of primary cultures from unerupted molars and incisors isolated from 5 to 7 days old pups. While primary cultures from both incisors and molars differentiated into odontoblasts and osteoblasts, cultures from molars differentiated into more DSPP-Cerulean + cells (?5.5 %) compared to incisor cultures (?0.7 %) at Day 14 and appear to be more committed to the odontogenic lineage. On the other hand, cultures from incisors show more differentiation into BSP-GFPtpz + cells (?25 %) compared to molar cultures (?16 %) and were more committed to the osteogenic lineage. Data were analyzed by Student's t-test and statistical significance was set at P ? 0.05. CONCLUSION:Since in the dental pulp, MSCs are the primary source of progenitors capable of giving rise to osteoblasts and odontoblasts, our results provide evidence for differences in the commitment of MSCs in molars and incisors to the odontogenic and osteogenic lineages, respectively.

SUBMITTER: Vijaykumar A 

PROVIDER: S-EPMC7050286 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature