Ontology highlight
ABSTRACT:
SUBMITTER: Sasmal DK
PROVIDER: S-EPMC7052167 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Sasmal Dibyendu K DK Feng Wei W Roy Sobhan S Leung Peter P He Yanran Y Cai Chufan C Cao Guoshuai G Lian Huada H Qin Jian J Hui Enfu E Schreiber Hans H Adams Erin J EJ Huang Jun J
Cellular & molecular immunology 20190917 3
A major unanswered question is how a TCR discriminates between foreign and self-peptides presented on the APC surface. Here, we used in situ fluorescence resonance energy transfer (FRET) to measure the distances of single TCR-pMHC bonds and the conformations of individual TCR-CD3ζ receptors at the membranes of live primary T cells. We found that a TCR discriminates between closely related peptides by forming single TCR-pMHC bonds with different conformations, and the most potent pMHC forms the s ...[more]