Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS.
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ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC7053107 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS.
Liu Yibin Y Cheng Jingfei J Siejka-Zielińska Paulina P Weldon Carika C Roberts Hannah H Lopopolo Maria M Magri Andrea A D'Arienzo Valentina V Harris James M JM McKeating Jane A JA Song Chun-Xiao CX
Genome biology 20200303 1
We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem ...[more]
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