Ontology highlight
ABSTRACT:
SUBMITTER: McIlwain BC
PROVIDER: S-EPMC7054162 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
McIlwain Benjamin C BC Martin Kamirah K Hayter Elizabeth A EA Stockbridge Randy B RB
Journal of molecular biology 20200114 4
Fluc family fluoride channels are assembled as primitive antiparallel homodimers. Crystallographic studies revealed a cation bound at the center of the protein, where it is coordinated at the dimer interface by main chain carbonyl oxygen atoms from the midmembrane breaks in two corresponding transmembrane helices. Here, we show that this cation is a stably bound sodium ion, and although it is not a transported substrate, its presence is required for the channel to adopt an open, fluoride-conduct ...[more]