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Dispersion preparation, characterization, and dosimetric analysis of cellulose nano-fibrils and nano-crystals: Implications for cellular toxicological studies.

ABSTRACT: The characterization of cellulose-based nanomaterial (CNM) suspensions in environmental and biological media is impaired because of their high carbon content and anisotropic shape, thus making it difficult to derive structure activity relationships (SAR) in toxicological studies. Here, a standardized method for the dispersion preparation and characterization of cellulose nanofibrils (CNF) and nanocrystals (CNC) in biological and environmental media was developed. Specifically, electron microscopy was utilized and allowed to specify optimum practices for efficiently suspending CNF and CNC in water and cell culture medium. Furthermore, a technique for measuring the in vitro particle kinetics of CNF and CNC suspended in cell culture medium utilizing fluorescently tagged materials was developed to assess the delivery rate of such CNM at the bottom of the well. Interestingly, CNF were shown to settle and create a loosely packed layer at the bottom of cell culture wells within a few hours. On the contrary, CNC settled gradually at a significantly slower rate, highlighting the discordance between administered and delivered mass dose. This work is both novel and urgent in the field of environmental health and safety as it introduces well-defined techniques for the dispersion and characterization of emerging, cellulose-based engineered nanomaterials. It also provides useful insights to the in vitro behavior of suspended anisotropic nanomaterials in general, which should enable dosimetry and comparison of toxicological data across laboratories as well as promote the safe and sustainable use of nanotechnology.

SUBMITTER: Bitounis D

PROVIDER: S-EPMC7055660 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Dispersion preparation, characterization, and dosimetric analysis of cellulose nano-fibrils and nano-crystals: Implications for cellular toxicological studies.

Bitounis Dimitrios D Pyrgiotakis Georgios G Bousfield Douglas D Demokritou Philip P

NanoImpact 20190301

The characterization of cellulose-based nanomaterial (CNM) suspensions in environmental and biological media is impaired because of their high carbon content and anisotropic shape, thus making it difficult to derive structure activity relationships (SAR) in toxicological studies. Here, a standardized method for the dispersion preparation and characterization of cellulose nanofibrils (CNF) and nanocrystals (CNC) in biological and environmental media was developed. Specifically, electron microscop ...[more]

PMID: 32133424

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Project description:Nano-enabled thermoplastics are part of the growing market of nano-enabled products (NEPs) that have vast utility in several industries and consumer goods. The use and disposal of NEPs at their end of life has raised concerns about the potential release of constituent engineered nanomaterials (ENMs) during thermal decomposition and their impact on environmental health and safety. To investigate this issue, industrially relevant nano-enabled thermoplastics including polyurethane, polycarbonate, and polypropylene containing carbon nanotubes (0.1 and 3% w/v, respectively), polyethylene containing nanoscale iron oxide (5% w/v), and ethylene vinyl acetate containing nanoscale titania (2 and 5% w/v) along with their pure thermoplastic matrices were thermally decomposed using the recently developed lab based Integrated Exposure Generation System (INEXS). The life cycle released particulate matter (called LCPM) was monitored using real time instrumentation, size fractionated, sampled, extracted and prepared for toxicological analysis using primary small airway epithelial cells to assess potential toxicological effects. Various cellular assays were used to assess reactive oxygen species and total glutathione as measurements of oxidative stress along with mitochondrial function, cellular viability, and DNA damage. By comparing toxicological profiles of LCPM released from polymer only (control) with nano-enabled LCPM, potential nanofiller effects due to the use of ENMs were determined. We observed associations between NEP properties such as the percent nanofiller loading, host matrix, and nanofiller chemical composition and the physico-chemical properties of released LCPM, which were linked to biological outcomes. More specifically, an increase in percent nanofiller loading promoted a toxicological response independent of increasing LCPM dose. Importantly, differences in host matrix and nanofiller composition were shown to enhance biological activity and toxicity of LCPM. This work highlights the importance of assessing the toxicological properties of LCPM and raises environmental health and safety concerns of nano-enabled products at their end of life during thermal decomposition/incineration.

Dataset Information

Dispersion preparation, characterization, and dosimetric analysis of cellulose nano-fibrils and nano-crystals: Implications for cellular toxicological studies.

Publications

Dispersion preparation, characterization, and dosimetric analysis of cellulose nano-fibrils and nano-crystals: Implications for cellular toxicological studies.

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