Ontology highlight
ABSTRACT:
SUBMITTER: Rallapalli KL
PROVIDER: S-EPMC7056309 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Rallapalli Kartik L KL Komor Alexis C AC Paesani Francesco F
Science advances 20200304 10
Adenine base editors, which were developed by engineering a transfer RNA adenosine deaminase enzyme (TadA) into a DNA editing enzyme (TadA*), enable precise modification of A:T to G⋮C base pairs. Here, we use molecular dynamics simulations to uncover the structural and functional roles played by the initial mutations in the onset of the DNA editing activity by TadA*. Atomistic insights reveal that early mutations lead to intricate conformational changes in the structure of TadA*. In particular, ...[more]