Dataset Information

The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles.

ABSTRACT:

Objective

To characterize the most relevant changes in the lipidome of endometrial fluid aspirate (EFA) in non-implantative cycles.

Design

Lipidomics in a prospective cohort study.

Settings

Reproductive unit of a university hospital.

Patients

Twenty-nine women undergoing an IVF cycle. Fifteen achieved pregnancy and 14 did not.

Intervention

Endometrial fluid aspiration immediately before performing embryo transfer.

Main outcome measures

Clinical pregnancy rate and lipidomic profiles obtained on an ultra-high performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-ToF-MS)-based analytical platform.

Results

The comparative analysis of the lipidomic patterns of endometrial fluid in implantative and non-implantative IVF cycles revealed eight altered metabolites: seven glycerophospholipids and an omega-6 polyunsaturated fatty acid. Then, women with a non-implantative cycle were accurately classified with a support vector machine algorithm including these eight lipid metabolites. The diagnostic performances of the algorithm showed an area under the receiver operating characteristic curve, sensitivity, specificity, and accuracy of 0.893?±?0.07, 85.7%, 80.0%, and 82.8%, respectively.

Conclusion

A predictive lipidomic signature linked to the implantative status of the endometrial fluid has been found.

SUBMITTER: Matorras R

PROVIDER: S-EPMC7056765 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Publications

The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles.

Matorras Roberto R Martinez-Arranz Ibon I Arretxe Enara E Iruarrizaga-Lejarreta Marta M Corral Blanca B Ibañez-Perez Jone J Exposito Antonia A Prieto Begoña B Elortza Felix F Alonso Cristina C

Journal of assisted reproduction and genetics 20191221 2

<h4>Objective</h4>To characterize the most relevant changes in the lipidome of endometrial fluid aspirate (EFA) in non-implantative cycles.<h4>Design</h4>Lipidomics in a prospective cohort study.<h4>Settings</h4>Reproductive unit of a university hospital.<h4>Patients</h4>Twenty-nine women undergoing an IVF cycle. Fifteen achieved pregnancy and 14 did not.<h4>Intervention</h4>Endometrial fluid aspiration immediately before performing embryo transfer.<h4>Main outcome measures</h4>Clinical pregnanc ...[more]

PMID: 31865491

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Project description:Introduction: Several authors have linked subclinical ovulatory disturbances in normal length menstrual cycles to premenopausal fracture risk and bone changes. This study systematically examined the influence of ovulation and anovulation on the bone metabolism of premenopausal women. Participants and Methods: In 176 cycles in healthy premenopausal women, FSH, 17β-estradiol (E2) and progesterone (P4) as well as bone alkalic phosphatase (BAP), pyridinoline (PYD) and C-terminal crosslinks (CTX) were measured during the follicular and during the luteal phase. The probability and timing of ovulation was self-assessed by a monitoring device. In addition, bone density of the lumbar spine was measured by quantitative computed tomography (QCT) at baseline and at the end of the study. Analysis was restricted to blood samples taken more than three days before the following menstruation. Results: 118 cycles out of the 176 collected cycles were complete with blood samples taken within the correct time interval. Of these, 56.8 % were ovulatory by two criteria (ovulation symbol shown on the monitor display and LP progesterone > 6 ng/ml), 33.1 % were possibly ovulatory by one criterion (ovulation symbol shown on the monitor display or LP progesterone > 6 ng/ml), and 10.2 % were anovulatory by both criteria). Ovulation in the previous cycle and in the same cycle did not significantly influence the mean absolute concentrations of the bone markers. However, bone formation (BAP) was higher in the luteal phase of ovulatory cycles than in anovulatory cycles (n. s.) and the relative changes within one cycle were significantly different for bone resorption (CTX) during ovulatory vs. anovulatory cycles (p < 0.01). In 68 pairs of cycles following each other directly, both ovulation in the previous cycle and ovulation in the present cycle influenced CTX, but not the differences of other bone markers. Conclusion: Ovulatory cycles reduce bone resorption in their luteal phase and that of the following cycle. The interaction between ovulation and bone metabolism is complex. Since anovulation may occur in low estrogen states such as pre-anorexic dietary restraint, as well as with high estrogenic circumstances e.g. from functional perimenopausal ovarian cysts, the association with bone changes has been variable in the literature. Accumulating physiological and clinical evidence however point towards a role for ovulation in enhancing bone formation and limiting bone resorption.

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Dataset Information

The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles.

Objective

Design

Settings

Patients

Intervention

Main outcome measures

Results

Conclusion

Publications

The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles.

Similar Datasets

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