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Controlled ovarian stimulation and progesterone supplementation affect vaginal and endometrial microbiota in IVF cycles: a pilot study.


ABSTRACT: PURPOSE:Does controlled ovarian stimulation (COS) and progesterone (P) luteal supplementation modify the vaginal and endometrial microbiota of women undergoing in vitro fertilization? METHODS:Fifteen women underwent microbiota analysis at two time points: during a mock transfer performed in the luteal phase of the cycle preceding COS, and at the time of fresh embryo transfer (ET). A vaginal swab and the distal extremity of the ET catheter tip were analyzed using next-generation 16SrRNA gene sequencing. Heterogeneity of the bacterial microbiota was assessed according to both the Bray-Curtis similarity index and the Shannon diversity index. RESULTS:Lactobacillus was the most prevalent genus in the vaginal samples, although its relative proportion was reduced by COS plus P supplementation (71.5?±?40.6% vs. 61.1?±?44.2%). In the vagina, an increase in pathogenic species was observed, involving Prevotella (3.5?±?8.9% vs. 12.0?±?19.4%), and Escherichia coli-Shigella spp. (1.4?±?5.6% vs. 2.0?±?7.8%). In the endometrium, the proportion of Lactobacilli slightly decreased (27.4?±?34.5% vs. 25.0?±?29.9%); differently, both Prevotella and Atopobium increased (3.4?±?9.5% vs. 4.7?±?7.4% and 0.7?±?1.5% vs. 5.8?±?12.0%). In both sites, biodiversity was greater after COS (p?

SUBMITTER: Carosso A 

PROVIDER: S-EPMC7492325 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Controlled ovarian stimulation and progesterone supplementation affect vaginal and endometrial microbiota in IVF cycles: a pilot study.

Carosso Andrea A   Revelli Alberto A   Gennarelli Gianluca G   Canosa Stefano S   Cosma Stefano S   Borella Fulvio F   Tancredi Annalisa A   Paschero Carlotta C   Boatti Lara L   Zanotto Elisa E   Sidoti Francesca F   Bottino Paolo P   Costa Cristina C   Cavallo Rossana R   Benedetto Chiara C  

Journal of assisted reproduction and genetics 20200715 9


<h4>Purpose</h4>Does controlled ovarian stimulation (COS) and progesterone (P) luteal supplementation modify the vaginal and endometrial microbiota of women undergoing in vitro fertilization?<h4>Methods</h4>Fifteen women underwent microbiota analysis at two time points: during a mock transfer performed in the luteal phase of the cycle preceding COS, and at the time of fresh embryo transfer (ET). A vaginal swab and the distal extremity of the ET catheter tip were analyzed using next-generation 16  ...[more]

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