Ontology highlight
ABSTRACT:
SUBMITTER: O'Brien SA
PROVIDER: S-EPMC7058118 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
O'Brien Sofie A SA Lee Kyoungho K Fu Hsu-Yuan HY Lee Zion Z Le Tung S TS Stach Christopher S CS McCann Meghan G MG Zhang Alicia Q AQ Smanski Michael J MJ Somia Nikunj V NV Hu Wei-Shou WS
Biotechnology journal 20180730 10
For the biomanufacturing of protein biologics, establishing stable cell lines with high transgene transcription is critical for high productivity. Modern genome engineering tools can direct transgene insertion to a specified genomic locus and can potentially become a valuable tool for cell line generation. In this study, the authors survey transgene integration sites and their transcriptional activity to identify characteristics of desirable regions. A lentivirus containing destabilized Green Fl ...[more]