Ontology highlight
ABSTRACT:
SUBMITTER: Douville C
PROVIDER: S-EPMC7060727 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Douville Christopher C Cohen Joshua D JD Ptak Janine J Popoli Maria M Schaefer Joy J Silliman Natalie N Dobbyn Lisa L Schoen Robert E RE Tie Jeanne J Gibbs Peter P Goggins Michael M Wolfgang Christopher L CL Wang Tian-Li TL Shih Ie-Ming IM Karchin Rachel R Lennon Anne Marie AM Hruban Ralph H RH Tomasetti Cristian C Bettegowda Chetan C Kinzler Kenneth W KW Papadopoulos Nickolas N Vogelstein Bert B
Proceedings of the National Academy of Sciences of the United States of America 20200219 9
We report a sensitive PCR-based assay called Repetitive Element AneupLoidy Sequencing System (RealSeqS) that can detect aneuploidy in samples containing as little as 3 pg of DNA. Using a single primer pair, we amplified ∼350,000 amplicons distributed throughout the genome. Aneuploidy was detected in 49% of liquid biopsies from a total of 883 nonmetastatic, clinically detected cancers of the colorectum, esophagus, liver, lung, ovary, pancreas, breast, or stomach. Combining aneuploidy with somatic ...[more]