Sensitivity boosts by the CPMAS CryoProbe for challenging biological assemblies.

ABSTRACT: Despite breakthroughs in MAS NMR hardware and experimental methodologies, sensitivity remains a major challenge for large and complex biological systems. Here, we report that 3-4 fold higher sensitivities can be obtained in heteronuclear-detected experiments, using a novel HCN CPMAS probe, where the sample coil and the electronics operate at cryogenic temperatures, while the sample is maintained at ambient temperatures (BioSolids CryoProbe™). Such intensity enhancements permit recording 2D and 3D experiments that are otherwise time-prohibitive, such as 2D ¹⁵N-¹⁵N proton-driven spin diffusion and ¹⁵N-¹³C double cross polarization to natural abundance carbon experiments. The benefits of CPMAS CryoProbe-based experiments are illustrated for assembli

SUBMITTER: Hassan A 

PROVIDER: S-EPMC7060763 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature