Ontology highlight
ABSTRACT:
SUBMITTER: Sullivan R
PROVIDER: S-EPMC7062242 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Sullivan Roisin R Heavey Susan S Graham David G DG Wellman Rachel R Khan Saif S Thrumurthy Sri S Simpson Benjamin S BS Baker Tina T Jevons Sarah S Ariza Jose J Eneh Victor V Pye Hayley H Luxton Hayley H Hamoudi Rifat R Whitaker Hayley H Lovat Laurence B LB
PloS one 20200309 3
Saliva represents an ideal matrix for diagnostic biomarker development as it is readily available and requires no invasive collection procedures. However, salivary RNA is labile and rapidly degrades. Previous attempts to isolate RNA from saliva have yielded poor quality and low concentrations. Here we compare collection and processing methods and propose an approach for future studies. The effects of RNA stabilisers, storage temperatures, length of storage and fasting windows were investigated o ...[more]