Unknown

Dataset Information

0

RHOA-mediated mechanical force generation through Dectin-1.


ABSTRACT: Dendritic cell-associated C-type lectin 1 (Dectin-1, also known as CLEC7A) is an innate immune pattern recognition receptor that recognizes ?-glucan on the Candida albicans cell wall. Recognition of ?-glucan by immune cells leads to phagocytosis, oxidative burst, cytokine and chemokine production. We looked for specific mechanisms that coordinate phagocytosis downstream of Dectin-1 leading to actin reorganization and internalization of fungus. We found that stimulation of Dectin-1 by soluble ?-glucan leads to mechanical force generation and areal contraction in Dectin-1-transfected HEK-293 cells and M1 macrophages. With inhibitor studies, we found this force generation is a spleen tyrosine kinase (SYK)-independent, but SRC family kinase (SFK)-dependent process mediated through the RHOA-ROCK-myosin light chain (MLC) pathway. We confirmed activation of RHOA downstream of Dectin-1 using activity assays and stress fiber formation. Through phagocytosis assays, we found direct evidence for the importance of RHOA-ROCK-MLC signaling in the process of phagocytosis of C. albicans.

SUBMITTER: Choraghe RP 

PROVIDER: S-EPMC7063837 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

RHOA-mediated mechanical force generation through Dectin-1.

Choraghe Rohan P RP   Kołodziej Tomasz T   Buser Alan A   Rajfur Zenon Z   Neumann Aaron K AK  

Journal of cell science 20200302 5


Dendritic cell-associated C-type lectin 1 (Dectin-1, also known as CLEC7A) is an innate immune pattern recognition receptor that recognizes β-glucan on the <i>Candida albicans</i> cell wall. Recognition of β-glucan by immune cells leads to phagocytosis, oxidative burst, cytokine and chemokine production. We looked for specific mechanisms that coordinate phagocytosis downstream of Dectin-1 leading to actin reorganization and internalization of fungus. We found that stimulation of Dectin-1 by solu  ...[more]

Similar Datasets

2017-12-23 | GSE93529 | GEO
| S-EPMC4721519 | biostudies-literature
2020-05-18 | GSE126735 | GEO
| S-EPMC6179142 | biostudies-literature
| S-EPMC6658619 | biostudies-literature
| S-EPMC3118584 | biostudies-literature
| S-EPMC5817926 | biostudies-literature
2011-01-14 | E-GEOD-26390 | biostudies-arrayexpress
| S-EPMC4165456 | biostudies-literature
| S-EPMC5626692 | biostudies-other