Organ Biodistribution of Radiolabelled ?? T Cells Following Liposomal Alendronate Administration in Different Mouse Tumour Models.

ABSTRACT: V?9V?2 T cell immunotherapy has been shown to be effective in delaying tumour growth in both pre-clinical and clinical studies. It has been pointed out the importance of the ability of cells to accumulate within tumours and the association with therapeutic efficacy in clinical studies of adoptive T cell transfer. We have previously reported that alendronate liposomes (L-ALD) increase the efficacy of this therapy after localised or systemic injection of ?? T cells in mice, inoculated with ovarian, melanoma, pancreatic or experimental lung metastasis tumour models, respectively. This study aimed to examine the organ biodistribution and tumour uptake of human ?? T cells in subcutaneous (SC), intraperitoneal (IP) or experimental metastatic lung tumours, established in NOD-SCID gamma (NSG) mice using the melanoma cell line A375P?6.luc. pre-injected with L-ALD. Overall, small variations in blood profiles and organ biodistribution of ?? T cells among the different tumour models were observed. Exceptionally, IP-tumour and experimental metastatic lung-tumour bearing mice pre-injected with L-ALD showed a significant decrease in liver accumulation, and highest uptake of ?? T cells in lungs and tumour-bearing lungs, respectively. Lower ?? T cell count was found in the SC and IP tumours.

SUBMITTER: Wang JT 

PROVIDER: S-EPMC7064741 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature