Unknown

Dataset Information

0

A portable, low-cost device for precise control of specimen temperature under stereomicroscopes.


ABSTRACT: To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably maintained at a precision of +/- 0.1?C. To demonstrate the utility of this device, we report improved characterization of the penetrance of a short-acting temperature-sensitive allele in C. elegans embryos, and identification of the upper temperature threshold for embryonic viability for six Caenorhabditis species. By controlling the temperature environment even as a specimen is manipulated, this device offers consistency and flexibility, reduces environmental noise, and enables precision timing in experiments requiring temperature shifts.

SUBMITTER: Testa ND 

PROVIDER: S-EPMC7065815 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

A portable, low-cost device for precise control of specimen temperature under stereomicroscopes.

Testa Nicholas D ND   Kaul Samiksha S   Le Kim N KN   Zhan Mei M   Lu Hang H   Paaby Annalise B AB  

PloS one 20200311 3


To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably mainta  ...[more]

Similar Datasets

| S-EPMC8099501 | biostudies-literature
| S-EPMC10058786 | biostudies-literature
2017-02-17 | GSE92495 | GEO
| S-EPMC10534372 | biostudies-literature
| S-EPMC6785052 | biostudies-literature
| S-EPMC9227208 | biostudies-literature
| S-EPMC6953602 | biostudies-literature
| S-EPMC5994145 | biostudies-literature
| S-EPMC9609715 | biostudies-literature