Advanced maternal age alters expression of maternal effect genes that are essential for human oocyte quality.
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ABSTRACT:
SUBMITTER: Zhang JJ
PROVIDER: S-EPMC7066876 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
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Advanced maternal age alters expression of maternal effect genes that are essential for human oocyte quality.
Zhang Jing-Jing JJ Liu Xiaoyan X Chen Li L Zhang Shouxin S Zhang Xia X Hao Cuifang C Miao Yi-Liang YL
Aging 20200225 4
To investigate the effects of maternal age on the quality of oocytes, we used single-cell RNA sequencing to detect global gene transcriptome and identify key genes affected by advanced age in human mature oocytes. We isolated mRNA from mature oocytes obtained from IVF or ICSI patients (three oocytes from younger (≤30 years) and three oocytes from older (≥40 years) patients for scRNA-seq. We identified 357 genes differentially expressed between matured oocytes from older and younger women's. The ...[more]
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