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Quantification of [11C]PBR28 data after systemic lipopolysaccharide challenge.


ABSTRACT: BACKGROUND:Lipopolysaccharide (LPS) is a classic immune stimulus. LPS combined with positron emission tomography (PET) 18?kDa translocator protein (TSPO) brain imaging provides a robust human laboratory model to study neuroimmune signaling. To evaluate optimal analysis of these data, this work compared the sensitivity of six quantification approaches. METHODS:[11C]PBR28 data from healthy volunteers (N = 8) were collected before and 3?h after LPS challenge (1.0?ng/kg IV). Quantification approaches included total volume of distribution estimated with two tissue, and two tissue plus irreversible uptake in whole blood, compartment models (2TCM and 2TCM-1k, respectively) and multilinear analysis-1 (MA-1); binding potential estimated with simultaneous estimation (SIME); standardized uptake values (SUV); and SUV ratio (SUVR). RESULTS:The 2TCM, 2TCM-1k, MA-1, and SIME approaches each yielded substantive effect sizes for LPS effects (partial ?2 = 0.56-0.89, ps <. 05), whereas SUV and SUVR did not. CONCLUSION:These findings highlight the importance of incorporating AIF measurements to quantify LPS-TSPO studies.

SUBMITTER: Woodcock EA 

PROVIDER: S-EPMC7067964 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Quantification of [<sup>11</sup>C]PBR28 data after systemic lipopolysaccharide challenge.

Woodcock Eric A EA   Schain Martin M   Cosgrove Kelly P KP   Hillmer Ansel T AT  

EJNMMI research 20200312 1


<h4>Background</h4>Lipopolysaccharide (LPS) is a classic immune stimulus. LPS combined with positron emission tomography (PET) 18 kDa translocator protein (TSPO) brain imaging provides a robust human laboratory model to study neuroimmune signaling. To evaluate optimal analysis of these data, this work compared the sensitivity of six quantification approaches.<h4>Methods</h4>[<sup>11</sup>C]PBR28 data from healthy volunteers (N = 8) were collected before and 3 h after LPS challenge (1.0 ng/kg IV)  ...[more]

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