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Helminth infection modulates systemic pro-inflammatory cytokines and chemokines implicated in type 2 diabetes mellitus pathogenesis.

ABSTRACT: BACKGROUND:The prevalence of helminth infections exhibits an inverse association with the prevalence of Type 2 diabetes mellitus (T2DM), and helminths are postulated to mediate a protective effect against T2DM. However, the biological mechanism behind this effect is not known. AIMS/METHODS:We postulated that helminth infections act by modulating the pro-inflammatory cytokine and chemokine milieu that is characteristic of T2DM. To examine the association of cytokines and chemokines in helminth-diabetes co-morbidity, we measured the plasma levels of a panel of pro-inflammatory cytokines and chemokines in individuals with Strongyloides stercoralis infection (Ss+) and T2DM at the time of Ss diagnosis and then 6 months after definitive anthelmintic treatment along with uninfected control individuals with T2DM alone (Ss-). PRINCIPAL FINDINGS:Ss+ individuals exhibited significantly diminished levels of the pro-inflammatory cytokines-IL-1?, IL-1?, IL-6, IL-12, IL-18, IL-23, IL-27, G-CSF and GM-CSF and chemokines-CCL1, CCL2, CCL3, CCL11, CXCL1, CXCL2, CXCL8, CXCL9, CXCL10 and CXCL11. In contrast, Ss+ individuals exhibited significantly elevated levels of IL-1Ra. Anthelmintic treatment resulted in increased levels of all of the cytokines and chemokines. CONCLUSIONS:Thus, helminth infections alleviate and anthelmintic therapy partially restores the plasma cytokine and chemokine levels in helminth-diabetes co-morbidity. Our data therefore offer a plausible biological mechanism for the protective effect of helminth infections against T2DM.

SUBMITTER: Rajamanickam A

PROVIDER: S-EPMC7069638 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Helminth infection modulates systemic pro-inflammatory cytokines and chemokines implicated in type 2 diabetes mellitus pathogenesis.

Rajamanickam Anuradha A Munisankar Saravanan S Dolla Chandrakumar C Menon Pradeep A PA Thiruvengadam Kannan K Nutman Thomas B TB Babu Subash S

PLoS neglected tropical diseases 20200303 3

<h4>Background</h4>The prevalence of helminth infections exhibits an inverse association with the prevalence of Type 2 diabetes mellitus (T2DM), and helminths are postulated to mediate a protective effect against T2DM. However, the biological mechanism behind this effect is not known.<h4>Aims/methods</h4>We postulated that helminth infections act by modulating the pro-inflammatory cytokine and chemokine milieu that is characteristic of T2DM. To examine the association of cytokines and chemokines ...[more]

PMID: 32126084

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Project description:Prostaglandin F2? (PGF2?) plays a critical role in the initiation and process of parturition. Since human labor has been described as an inflammatory event, we investigated the role of PGF2? in the inflammatory process using cultured human uterine smooth muscle cells (HUSMCs) isolated from term pregnant women as a model. Using a multiplex assay, HUSMCs treated with PGF2? changed their output of a number of cytokines and chemokines, with a distinct response pattern that differed between HUSMCs isolated from the upper and lower segment region of the uterus. Confirmatory enzyme-linked immunosorbent assays (ELISAs) showed that PGF2? stimulated increased output of interleukin (IL) 1?, IL6, IL8 (CXCL8) and monocyte chemotactic protein-1 (MCP1, also known as chemokine (c-c motif) ligand 2, CCL2) by HUSMCs isolated from both upper and lower uterine segments. In contrast, PGF2? inhibited tumor necrosis factor ? (TNF?) release by HUMSCs from the lower uterine segment while the output of TNF? was undetectable in the upper segment. Small interfering (si) RNA mediated knockdown of the PGF2? receptor prevented the changes in cytokine and chemokine output by the HUSMCs. Since the PGF2? receptor (PTGFR) couples via the Gq protein and subsequently activates the phospholipase C (PLC) and protein kinase C (PKC) signaling pathways, we examined the role of these pathways in PGF2? modulation of the cytokines. Inhibition of PLC and PKC reversed the effects of PGF2?. PGF2? activated multiple signaling pathways including extracellular signal-regulated kinases (ERK) 1/2, phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K), P38, calcineurin/nuclear factor of activated T-cells (NFAT) and NF-?B signaling. Inhibition of ERK reversed PGF2?-induced IL1?, IL6 and CCL2 output, while inhibition of PI3K blocked the effect of PGF2? on IL6, CXCL8 and CCL2 output and inhibition of NF-?B reversed PGF2?-induced IL1? and CCL2 output. NFAT was involved in PGF2? modulation of CCL2 and TNF? output. In conclusion, our results support a role of PGF2? in creating an inflammatory environment during the late stage of human pregnancy.

Dataset Information

Helminth infection modulates systemic pro-inflammatory cytokines and chemokines implicated in type 2 diabetes mellitus pathogenesis.

Publications

Helminth infection modulates systemic pro-inflammatory cytokines and chemokines implicated in type 2 diabetes mellitus pathogenesis.

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