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Enzymatic production of 4-O-methyl d-glucaric acid from hardwood xylan.


ABSTRACT: Background:Dicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4-O-methyl d-glucaric acid, could potentially be produced from glucuronoxylans, which are a comparatively underused fraction of wood and agricultural biorefineries. Results:Accordingly, an enzymatic pathway was developed that combines AxyAgu115A, a GH115 ?-glucuronidase from Amphibacillus xylanus, and GOOX, an AA7 gluco-oligosaccharide oxidase from Sarocladium strictum, to produce this bio-based chemical from glucuronoxylan. AxyAgu115A was able to release almost all 4-O-methyl d-glucuronic acid from glucuronoxylan while a GOOX variant, GOOX-Y300A, could convert 4-O-methyl d-glucuronic acid to the corresponding glucaric acid at a yield of 62%. Both enzymes worked effectively at alkaline conditions that increase xylan solubility. Given the sensitivity of AxyAgu115A to hydrogen peroxide and optimal performance of GOOX-Y300A at substrate concentrations above 20 mM, the two-step enzyme pathway was demonstrated as a sequential, one-pot reaction. Additionally, the resulting xylan was easily recovered from the one-pot reaction, and it was enzymatically hydrolysable. Conclusions:The pathway in this study requires only two enzymes while avoiding a supplementation of costly cofactors. This cell-free approach provides a new strategy to make use of the underutilized hemicellulose stream from wood and agricultural biorefineries.

SUBMITTER: Vuong TV 

PROVIDER: S-EPMC7071571 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Enzymatic production of 4-<i>O</i>-methyl d-glucaric acid from hardwood xylan.

Vuong Thu V TV   Master Emma R ER  

Biotechnology for biofuels 20200313


<h4>Background</h4>Dicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4-<i>O</i>-methyl d-glucaric acid, could potentially be produced from glucuronoxylans, which are a comparatively underused fraction of wood and agricultural biorefineries.<h4>Results</h4>Accordingly, an enzymatic pathway was developed that combines AxyAgu115A, a GH115 α-glucuronidase from <i>Amphibacillus xylanus</i>, and GOOX, an AA7 gluco-oligosaccharide oxidase from  ...[more]

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