Dataset Information

Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus.

ABSTRACT: The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid mycorrhizal (ERM) fungi, the molecular and cellular mechanisms that extend tolerance to the host plant are currently unknown. Here, we show a reduced Cd content in Cd-exposed mycorrhizal roots of Vaccinium myrtillus colonized by a metal tolerant isolate of the fungus Oidiodendron maius as compared to non-mycorrhizal roots. To better understand this phenotype, we applied Next Generation Sequencing technologies to analyze gene expression in V. myrtillus and O. maius Zn grown under normal and Cd-stressed conditions, in the free living and in the mycorrhizal status. The results clearly showed that Cd had a stronger impact on plant gene expression than symbiosis, whereas fungal gene expression was mainly regulated by symbiosis. The higher abundance of transcripts coding for stress related proteins in non-mycorrhizal roots may be related to the higher Cd content. Regulated plant metal transporters have been identified that may play a role in reducing Cd content in mycorrhizal roots exposed to this metal.

SUBMITTER: Casarrubia S

PROVIDER: S-EPMC7075258 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal <i>Vaccinium myrtillus</i>.

Casarrubia Salvatore S Martino Elena E Daghino Stefania S Kohler Annegret A Morin Emmanuelle E Khouja Hassine-Radhouane HR Murat Claude C Barry Kerrie W KW Lindquist Erika A EA Martin Francis M FM Perotto Silvia S

Frontiers in microbiology 20200309

The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid mycorrhizal (ERM) fungi, the molecular and cellular mechanisms that extend tolerance to the host plant are currently unknown. Here, we show a reduced Cd content in Cd-exposed mycorrh ...[more]

PMID: 32210940

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Project description:Abstract Climate change is altering the world's ecosystems through direct effects of climate warming and precipitation changes but also indirectly through changes in biotic interactions. For instance, climate‐driven changes in plant and/or insect communities may alter plant–pollinator interactions, thereby influencing plant reproductive success and ultimately population dynamics of insect‐pollinated plants. To better understand how the importance of insect pollination for plant fruit set varies with climate, we experimentally excluded pollinators from the partly selfing keystone species Vaccinium myrtillus along elevational gradients in the forest‐tundra ecotone in central Norway. The study comprised three mountain areas, seven elevational gradients spanning from the climatically relatively benign birch forest to the colder alpine areas above the tree line, and 180 plots of 1 × 1 m, with experimental treatments allocated randomly to plots within sites. Within the experimental plots, we counted the number of flowers of V. myrtillus and counted and weighed all fruits, as well as seeds for a selection of fruits. Excluding pollinators resulted in lower fruit production, as well as reduced fruit and seed mass of V. myrtillus. In the alpine sites pollinator exclusion resulted in 84% fewer fruits, 50% lower fruit weight, and 50% lower seed weight compared to control conditions. Contrary to our expectations, the negative effect of pollinator exclusion was less pronounced in the forest compared to alpine sites, suggesting that the importance of insect pollination for seed production is lower at low elevations. Our findings indicate that the keystone species V. myrtillus is relatively robust to changes in the pollinator community in a warmer climate, thereby making it less vulnerable to climate‐driven changes in plant–pollinator interactions. Climate‐driven changes in plant and/or insect communities may alter plant–pollinator interactions in a warming world, thereby influencing plant reproductive success and ultimately population dynamics of insect‐pollinated plants. Our experimental study shows that the plant species Vaccinium myrtillus is more robust to changes in the pollinator community in warmer compared to colder climates. This suggests that this keystone species is less vulnerable to climate‐driven changes in plant–pollinator interactions than expected.

Dataset Information

Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus.

Publications

Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal <i>Vaccinium myrtillus</i>.

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