Chicken eEF1? is a Critical Factor for the Polymerase Complex Activity of Very Virulent Infectious Bursal Disease Virus.

ABSTRACT: Infectious bursal disease (IBD) is an immunosuppressive, highly contagious, and lethal disease of young chickens caused by IBD virus (IBDV). It results in huge economic loss to the poultry industry worldwide. Infection caused by very virulent IBDV (vvIBDV) strains results in high mortality in young chicken flocks. However, the replication characteristics of vvIBDV are not well studied. Publications have shown that virus protein 3 (VP3) binds to VP1 and viral double-stranded RNA, and together they form a ribonucleoprotein complex that plays a key role in virus replication. In this study, vvIBDV VP3 was used to identify host proteins potentially involved in modulating vvIBDV replication. Chicken eukaryotic translation elongation factor 1? (cheEF1?) was chosen to further investigate effects on vvIBDV replication. By small interfering RNA-mediated cheEF1? knockdown, we demonstrated the possibility of significantly reducing viral polymerase activity, with a subsequent reduction in virus yields. Conversely, over-expression of cheEF1? significantly increased viral polymerase activity and virus replication. Further study confirmed that cheEF1? interacted only with vvIBDV VP3 but not with attenuated IBDV (aIBDV) VP3. Furthermore, the amino acids at the N- and C-termini were important in the interaction between vvIBDV VP3 and cheEF1?. Domain III was essential for interactions between cheEF1? and vvIBDV VP3. In summary, cheEF1? enhances vvIBDV replication by promoting the activity of virus polymerase. Our study indicates cheEF1? is a potential target for limiting vvIBDV infection.

SUBMITTER: Yang B 

PROVIDER: S-EPMC7077273 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature