Multiplex Screening Assay for Identifying Cytotoxic CD8⁺ T Cell Epitopes.

ABSTRACT: The cytotoxicity of epitope-specific CD8⁺ T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8⁺ T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for a systematic screening of CD8⁺ T cell epitopes. In addition, this greatly enhanced capacity enables the incorporation of irrelevant epitopes for determining the non-specific killing activity of CD8⁺ T cells, thereby allowing to measure the actual epitope-specific cytotoxicity

SUBMITTER: Poh CM 

PROVIDER: S-EPMC7078160 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature