Ontology highlight
ABSTRACT:
SUBMITTER: Poh CM
PROVIDER: S-EPMC7078160 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Poh Chek Meng CM Zheng Jian J Channappanavar Rudragouda R Chang Zi Wei ZW Nguyen Thi H O THO Rénia Laurent L Kedzierska Katherine K Perlman Stanley S Poon Leo L M LLM
Frontiers in immunology 20200311
The cytotoxicity of epitope-specific CD8<sup>+</sup> T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8<sup>+</sup> T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for ...[more]