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Zein-layered hydroxide biohybrids: strategies of synthesis and characterization.

ABSTRACT: This work constitutes a basic study about the first exploration on the preparation of biohybrids based on the corn protein zein and layered metal hydroxides, such as layered double hydroxides (LDH) and layered single hydroxides (LSHs). For this purpose, MgAl layered double hydroxide and the Co2(OH)3 layered single hydroxide were selected as hosts, and various synthetic approaches were explored to achieve the formation of the zein-layered hydroxide biohybrids, profiting from the presence of negatively charged groups in zein in basic medium. Zein-based layered hydroxide biohybrids were characterized by diverse physicochemical techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis/differential thermal analysis (TG/DTA), solid state 13C cross-polarization magical angle spinning nuclear magnetic resonance (CP-MAS NMR), field emission-scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), etc., which suggest that the different synthesis procedures employed and the anion located in the interlayer region of the inorganic host material seem to have a strong influence on the final features of the biohybrids, resulting in mixed, single intercalated, or highly exfoliated intercalated phases. Thus, the resulting biohybrids based on zein and layered hydroxides could have interest in applications in biomedicine, biosensing, materials for electronic devices, catalysis, and photocatalysis.

SUBMITTER: Alcantara ACS

PROVIDER: S-EPMC7079599 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Zein-layered hydroxide biohybrids: strategies of synthesis and characterization.

Alcântara Ana C S ACS Darder Margarita M Aranda Pilar P Ruiz-Hitzky Eduardo E

Materials (Basel, Switzerland) 20200211 4

This work constitutes a basic study about the first exploration on the preparation of biohybrids based on the corn protein zein and layered metal hydroxides, such as layered double hydroxides (LDH) and layered single hydroxides (LSHs). For this purpose, MgAl layered double hydroxide and the Co<sub>2</sub>(OH)<sub>3</sub> layered single hydroxide were selected as hosts, and various synthetic approaches were explored to achieve the formation of the zein-layered hydroxide biohybrids, profiting from ...[more]

PMID: 32054135

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Project description:2 : 1 Co/Ti layered double hydroxide (LDH) was synthesized hydrothermally using commercially available Co(NO3)2·6H2O and TiCl4, on a urea template. The high surface area material (~180 m2 g-1) had a narrow band gap (2.67 eV) and shallow and deep trap defect sites. The layered nanomaterial exhibited remarkable semiconductor properties and demonstrated excellent visible light decolourisation efficiency for the anionic dye Congo Red in aqueous medium. The photocatalytic efficiency of the LDH was better than common commercial materials in use such as ZnO, ZnS, NiO, CoO, TiO2 and Degussa P25. The presence of different surface states of defect sites in the LDH was confirmed by PL, EIS and XPS measurements. XRD, DRS, FT-IR, AFM, TEM, SEM/EDX and TG/DTG analyses yielded information about the structural, morphological properties and thermal stability of the LDH. BET N2 adsorption-desorption measurements at 77 K gave surface area and porosity data for the LDH. The surface charge characteristics of the LDH were evaluated with ξ-potential measurements over a wide pH-range in aqueous medium. The photocatalytic behaviour towards decolourisation of the dye was evaluated depending on the reaction variables of pH, LDH amount, initial dye concentration and effects of quenchers, and variation of molar ratios of Co/Ti LDH. The pseudo-first order model satisfactorily described the degradation kinetics of the anionic dye. The photocatalytic mechanistic pathways of the LDH were explained on the basis of an electron-hole (e--h+) hopping conduction model and also photosensitization of the dye. The maximum catalytic efficiency was observed with 15.0 mg of LDH at pH 4 for the anionic Congo Red dye at a concentration 1 × 10-5 M. The LDH was stable even after the fifth catalytic cycle, indicating its remarkable efficiency in potential decolourisation treatments. The dye degradation products were analysed with GC-MS and a reaction mechanism was proposed for the breakdown of the dye to simple and less toxic components.

Project description:BackgroundLayered double hydroxide lactate nanosheets (LDH-lactate-NS) are powerful carriers for delivering macro-molecules into intact plant cells. In the past few years, some studies have been carried out on DNA/RNA transformation and plant disease resistance, but little attention has been paid to these factors during LDH-lactate-NS synthesis and delamination, nor has their relationship to the DNA adsorption capacity or transformation efficiency of plant cells been considered.ResultsSince the temperature during delamination alters particle sizes and zeta potentials of LDH-lactate-NS products, we compared the LDH-lactate-NS stability, DNA adsorption rate and delivery efficiency of fluorescein isothiocyanate isomer I (FITC) of them, found that the LDH-lactate-NS obtained at 25 °C has the best characters for delivering biomolecules into plant cell. To understand the potential side effects and cytotoxicity of LDH-lactate-NS to plants, we compared the root growth rate between the Arabidopsis thaliana seedlings grown in the culture medium with 1-300 μg/mL LDH-lactate-NS and equivalent raw material, Mg(lactate)2 and Al (lactate)3. Phenotypic analysis showed LDH in a range of 1-300 μg/mL can enhance the root elongation, whereas the same concentration of raw materials dramatically inhibited root elongation, suggesting the nanocrystallization has a dramatical de-toxic effect to Mg(lactate)2 and Al (lactate)3. Since enhancing of root elongation by LDH is an unexpected phenomenon, we further designed experiments to investigate influence of LDH to Arabidopsis seedlings. We further used the gravitropic bending test, qRT-PCR analysis of auxin transport proteins, non-invasive micro-test technology and liquid chromatography-mass spectrometry to investigate the auxin transport and distribution in Arabidopsis root. Results indicated that LDH-lactate-NS affect root growth by increasing the polar auxin transport.ConclusionsOptimal synthesized LDH-lactate-NS can delivery biomolecules into intact plant cells with high efficiency and low cytotoxity. The working solution of LDH-lactate-NS can promote root elongation via increase the polar auxin transport in Arabidopsis roots.

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Zein-layered hydroxide biohybrids: strategies of synthesis and characterization.

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Zein-layered hydroxide biohybrids: strategies of synthesis and characterization.

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