Ontology highlight
ABSTRACT:
SUBMITTER: Morita S
PROVIDER: S-EPMC7084704 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Morita Sumiyo S Horii Takuro T Kimura Mika M Hatada Izuho I
International journal of molecular sciences 20200225 5
Overexpression of a gene of interest is a general approach used in both basic research and therapeutic applications. However, the conventional approach involving overexpression of exogenous genes has difficulty achieving complete genome coverage, and is also limited by the cloning capacity of viral vectors. Therefore, an alternative approach would be to drive the expression of an endogenous gene using an artificial transcriptional activator. Fusion proteins of dCas9 and a transcription activatio ...[more]