Ontology highlight
ABSTRACT:
SUBMITTER: Javaid N
PROVIDER: S-EPMC7795359 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Javaid Nasir N Pham Thuong L H TLH Choi Sangdun S
International journal of molecular sciences 20210101 1
Reversal in the transcriptional status of desired genes has been exploited for multiple research, therapeutic, and biotechnological purposes. CRISPR/dCas9-based activators can activate transcriptionally silenced genes after being guided by gene-specific gRNA(s). Here, we performed a functional comparison between two such activators, VP64-dCas9-VP64 and dCas9-VP192, in human embryonic kidney cells by the concomitant targeting of <i>POU5F1</i> and <i>SOX2</i>. We found 22- and 6-fold upregulations ...[more]