Ontology highlight
ABSTRACT:
SUBMITTER: Fukuda M
PROVIDER: S-EPMC7086690 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Fukuda Masaharu M Kuga Kazufumi K Miyazaki Ayako A Suzuki Tohru T Tasei Keito K Aita Tsunehiko T Mase Masaji M Sugiyama Makoto M Tsunemitsu Hiroshi H
Archives of virology 20120311 6
A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine coronavirus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10(2), 10(0), 10(1), and 10(2) TCID(50)/ml, respectively, and that for GBR was 10(6) copies/ml. The one-step m ...[more]