Unknown

Dataset Information

0

Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR.


ABSTRACT: Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats.

SUBMITTER: Noh JY 

PROVIDER: S-EPMC7086956 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR.

Noh Ji Yeong JY   Yoon Sun-Woo SW   Kim Doo-Jin DJ   Lee Moo-Seung MS   Kim Ji-Hyung JH   Na Woonsung W   Song Daesub D   Jeong Dae Gwin DG   Kim Hye Kwon HK  

Archives of virology 20170220 6


Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SAR  ...[more]

Similar Datasets

| S-EPMC3911421 | biostudies-literature
| S-EPMC7126732 | biostudies-literature
| S-EPMC7169817 | biostudies-literature
| S-EPMC6002729 | biostudies-literature
| S-EPMC4940488 | biostudies-literature
| S-EPMC3810765 | biostudies-literature
| S-EPMC7155742 | biostudies-literature
| S-EPMC4721578 | biostudies-literature
| S-EPMC5362064 | biostudies-literature
| S-EPMC4773072 | biostudies-literature