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Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay.

ABSTRACT: An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5' untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.

SUBMITTER: Yang J

PROVIDER: S-EPMC7087303 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay.

Yang Jin J Fang Mei-xin MX Li Jie J Lou Guo-qiang GQ Lu Hang-jun HJ Wu Nan-ping NP

Archives of virology 20110512 8

An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5' untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletropho ...[more]

PMID: 21562883

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