Ontology highlight
ABSTRACT:
SUBMITTER: Qiao N
PROVIDER: S-EPMC7058275 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Qiao Ning N Dai Huijie H Liu Jie J Zhu Xiaoping X Li Jintang J Zhang Dezhen D Liu Yongguang Y
PloS one 20200305 3
Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV. A set of four LAMP primers was designed based on the coat protein gene sequence of MNSV, and a RT-LAMP reaction was successfully performed for 1 h at 62°C. The results of RT-LAMP showed high specificity for MNSV and no cross-reaction with other viruses. ...[more]