Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input.
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ABSTRACT:
SUBMITTER: Daigneault BW
PROVIDER: S-EPMC7092705 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
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Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input.
Daigneault Bradford W BW Rajput Sandeep K SK Smith George W GW
BioTechniques 20200115 3
We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and agriculture, including clinical diagnoses of infertility, the identification of single-nucleotide polymorphisms and aberrant methylation patterns that can impact fertility, lower embryo development an ...[more]
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