Dataset Information

Serum procalcitonin concentrations in dogs with induced endotoxemia.

ABSTRACT:

Background

Procalcitonin (PCT) is an important biomarker for sepsis in human medicine, but there is little information regarding PCT as a biomarker for sepsis in dogs. There are no controlled studies evaluating serial concentrations of PCT in dogs.

Hypothesis/objective

That PCT would be rapidly detectable in serum after injection of LPS and would remain increased for at least 24?hours. Objective was to evaluate serial serum PCT concentrations in dogs after a single IV injection of LPS compared to placebo.

Animals

Six healthy mixed breed dogs.

Methods

A nonrandomized, placebo-controlled, crossover study was performed. Dogs were initially injected with placebo (0.9% NaCl; 1 mL, IV) and then experimental endotoxemia was induced by injecting lipopolysaccharide (LPS; 2 ?g/kg, IV, once) after a 5-day washout period. Serial blood samples were collected for measurement of serum PCT after each injection. Difference in median PCT concentration between serial time points was assessed using a mixed effects model.

Results

After LPS administration, blood pressure decreased and body temperature increased along with the development of lethargy, vomiting, and diarrhea. Procalcitonin was significantly increased compared to baseline by 2 hours after injection of LPS (median = 67.9 versus 172.8, range = 46.0-74.1 versus 99.5-295.9, P = .0002) and remained significantly increased for 12?hours (median = 205.9, range = 119.9-297.4) with return to baseline by 48?hours. Procalcitonin was significantly higher than placebo 2, 4, 6, 8, 10, 12, and 24?hours after injection. There were no significant differences in PCT between time 0 and any of the subsequent time points in the saline group.

Conclusions and clinical importance

Procalcitonin expression is likely to be a clinically useful biomarker for sepsis in dogs and might have an additional role in prognostication and therapeutic decision-making.

SUBMITTER: Easley F

PROVIDER: S-EPMC7096631 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Publications

Serum procalcitonin concentrations in dogs with induced endotoxemia.

Easley Frankie F Holowaychuk Marie K MK Lashnits Erin W EW Nordone Shila K SK Marr Henry H Birkenheuer Adam J AJ

Journal of veterinary internal medicine 20200122 2

<h4>Background</h4>Procalcitonin (PCT) is an important biomarker for sepsis in human medicine, but there is little information regarding PCT as a biomarker for sepsis in dogs. There are no controlled studies evaluating serial concentrations of PCT in dogs.<h4>Hypothesis/objective</h4>That PCT would be rapidly detectable in serum after injection of LPS and would remain increased for at least 24 hours. Objective was to evaluate serial serum PCT concentrations in dogs after a single IV injection of ...[more]

PMID: 31970837

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Dataset Information

Serum procalcitonin concentrations in dogs with induced endotoxemia.

Background

Hypothesis/objective

Animals

Methods

Results

Conclusions and clinical importance

Publications

Serum procalcitonin concentrations in dogs with induced endotoxemia.

Similar Datasets

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